Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
نویسندگان
چکیده
منابع مشابه
Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorp...
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AIMS To detect a wide range of Cryptosporidium species from human faeces by analysis of the Cryptosporidium oocyst wall protein gene by PCR. METHODS AND RESULTS The nested-assay comprised an initial amplification using a conventional thermocycler followed by real time PCR using a LightCycler with SYBR Green I for the characterization of the amplicons. The technique uses four sets of primers c...
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ژورنال
عنوان ژورنال: The Korean Journal of Parasitology
سال: 2013
ISSN: 1738-0006
DOI: 10.3347/kjp.2013.51.3.353